vector control pcdna-nc  (Genechem)

Journal: American Journal of Translational Research

Article Title: TUG1 aggravates intracerebral hemorrhage injury by inhibiting angiogenesis in an miR-26a-dependent manner

doi:

Figure Lengend Snippet: Roles of TUG1 or miR-26a in neurological deficits in rats with ICH. (A) pcDNA-TUG1 expression in vivo. (B-D) The effects of TUG1 overexpression on mNSS in rats on the 1st (B), 3rd (C), and 7th (D) day after ICH. (E) The expression of miR-26a mimics in rats. (F-H) The effects of miR-26a mimics on mNSS in ICH rats on the 1st (F), 3rd (G) and 7th (H) day after ICH. ***P < 0.001, vs. sham; #P < 0.05, vs. pcDNA-NC; ##P < 0.01, vs. pcDNA-NC; &P < 0.05, vs. mimic-NC; &&P < 0.01, vs. mimic-NC; &&&P < 0.001, vs. mimic-NC.

Article Snippet: The design and construction of recombinant plasmids, including TUG1-expressing pcDNA-TUG1 and vector control pcDNA-NC, were done by Genechem (Shanghai, China).

Techniques: Expressing, In Vivo, Over Expression

Journal: American Journal of Translational Research

Article Title: TUG1 aggravates intracerebral hemorrhage injury by inhibiting angiogenesis in an miR-26a-dependent manner

doi:

Figure Lengend Snippet: Effects of TUG1 or miR-26a on cerebral angiogenesis in rats with ICH. A. VEGF mRNA levels in ICH rats treated with mimic-NC or miR-26a mimics. B. The expression of VEGF and CD31 in ICH rats injected with miR-26a mimics. Original magnification, × 400. C. VEGF mRNA levels in ICH rats injected with pcDNA-NC or pcDNA-TUG1. D. IHC was performed to detect the expression of VEGF and CD31 in ICH rats injected with pcDNA-TUG1. Original magnification, × 400. ***P < 0.001, vs. sham; &&P < 0.01, vs. mimic-NC; ###P < 0.001, vs. pcDNA-NC.

Article Snippet: The design and construction of recombinant plasmids, including TUG1-expressing pcDNA-TUG1 and vector control pcDNA-NC, were done by Genechem (Shanghai, China).

Techniques: Expressing, Injection

Journal: American Journal of Translational Research

Article Title: TUG1 aggravates intracerebral hemorrhage injury by inhibiting angiogenesis in an miR-26a-dependent manner

doi:

Figure Lengend Snippet: TUG1 directly interacts with miR-26a. A. The miR-26a-binding site on TUG1 was analyzed. B. The WT-TUG1 or MUT-TUG1 luciferase reporter activities were analyzed. C. miR-26a expression in ICH rats treated with pcDNA-TUG1. &&&P < 0.001, vs. mimic-NC; ***P < 0.001, vs. sham; ###P < 0.001, vs. pcDNA-NC.

Article Snippet: The design and construction of recombinant plasmids, including TUG1-expressing pcDNA-TUG1 and vector control pcDNA-NC, were done by Genechem (Shanghai, China).

Techniques: Binding Assay, Luciferase, Expressing

Journal: American Journal of Translational Research

Article Title: TUG1 aggravates intracerebral hemorrhage injury by inhibiting angiogenesis in an miR-26a-dependent manner

doi:

Figure Lengend Snippet: TUG1 overexpression abolished the roles of miR-26a in the neurobehavioral recovery and the cerebral angiogenesis of ICH rats. (A-C) The effects of TUG1 overexpression on mNSS in rats treated with miR-26a mimics on the 1st (A), 3rd (B) and 7th (C) day after ICH. (D) pcDNA-TUG1 attenuated the promoting effect of miR-26a on VEGF mRNA level. (E) The expression of VEGF and CD31 in ICH rats treated with miR-26a mimics + pcDNA-NC or miR-26a mimics + pcDNA-TUG1. Original magnification, × 400. (F) Western blot of VEGF and CD31 expression in different groups. ##P < 0.01, vs. pcDNA-NC.

Article Snippet: The design and construction of recombinant plasmids, including TUG1-expressing pcDNA-TUG1 and vector control pcDNA-NC, were done by Genechem (Shanghai, China).

Techniques: Over Expression, Expressing, Western Blot

Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

doi: 10.3892/etm.2021.10512

Figure Lengend Snippet: miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by regulating E2F3 expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and pcDNA-E2F3 were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.

Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

Techniques: Migration, Expressing, Over Expression, Western Blot, Transfection, Cell Counting, Quantitative RT-PCR, Negative Control